Effects of 17β-Estradiol on the Expression of IL-6, IL-11 and NF-κB in Human MG-63 Osteoblast-like C

• 作 者: 王运林 廖二元 向光大 戴如春 肖新华 罗湘杭
• 机 构: Department of Gerontism Tongji Hospital Tongji Medical College Huazhong university of Science and T
• 出 处: 华中科技大学学报(医学英德文版)
• 发表日期: 2006年01期
• 关键词: estrogen osteoclast polyclonal osteosarcoma exact pression bands annealing ascor

In order to characterize the effects of 17β-estradiol (17β-E_2) on the expression of IL-6, IL-11 and NF-κB in the human MG-63 osteoblast-like cell line, the expression of IL-6 was detected by RT-PCR, Northern blot and Western blot. The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E_2 down-regulated the expression of IL-6 mRNA and protein, IL-11 mRNA and NF-κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17β-E_2, and this might lend support to estrogen replacement therapy in postmenopausal women.

In order to characterize the effects of 17β-estradiol (17β-E_2) on the expression of IL-6, IL-11 and NF-κB in the human MG-63 osteoblast-like cell line, the expression of IL-6 was detected The expression of IL-11 was determined by RT-PCR, and NF-κB by Western blot. The results showed that 17β-E_2 down-regulated the expression of IL-6 mRNA and It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17β-E_2, and this might lend support to estrogen replacement therapy in postmenopausal women.