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以甜叶菊甙提取液为试验原料,研究模拟移动床色谱法分离甜叶菊甙中各因素对甜叶菊甙纯度和收率的影响。通过单因素和四元二次回归正交旋转组合试验,优化出模拟移动床色谱法分离甜叶菊甙的最佳工艺参数,即:切换时间12 min、进样质量浓度40 mg/m L、进料流速7.5 m L/min、水洗1流速10 m L/min、解吸流速15.4m L/min、再生流速7.26 m L/min、水洗2流速20.3 m L/min。各区域最优分配及连接方式:吸附区5根色谱柱三并两串,水洗1区3根色谱柱串联,解吸区6根色谱柱串联,再生区3根色谱柱一串两并,逆向进料,水洗2区3根色谱柱串联。此条件下所得甜叶菊甙纯度为80.69%,收率为93.48%。
Taking rebaudioside extract as raw material, the effects of various factors on the purity and yield of rebaudioside by simulating the separation of rebaudioside by moving bed chromatography were studied. The optimum technological parameters for the separation of rebaudioside by simulated moving bed chromatography were optimized by single factor and quaternary quadratic regression orthogonal rotation combination test, ie, the switching time was 12 min, the injection mass concentration was 40 mg / m L, The flow rate was 7.5 m L / min, the flow rate of washing 1 was 10 m L / min, the desorption flow rate was 15.4 m L / min, the regeneration flow rate was 7.26 m L / min and the flow rate of water was 2 20.3 m L / min. The optimal distribution and connection of each region: five columns in the adsorption zone are three and two strings, one zone is washed in series, three columns are connected in series, the six columns in the desorption zone are connected in series, the three columns in the regeneration zone are connected in series and in reverse Materials, washed 2 District 3 columns in series. Under the conditions, the purity of the obtained rebaudioside was 80.69% and the yield was 93.48%.