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用Ｗｅｓｔｅｒｎ免疫印迹技术检测生长激素对大鼠肾脏微粒体细胞色素Ｐ４５０４Ａ２的影响。大鼠肾微粒体细胞色素Ｐ４５０与肝脏的一样，有种属、性别差异特异性。正常雄性大鼠肾微粒体细胞色素Ｐ４５０４Ａ２含量大于雌性大鼠的含量。切除垂体的雄性和雌性大鼠肾微粒体细胞色素Ｐ４５０４Ａ２含量明显高于正常对照大鼠的水平，而雌、雄性之间的差异已不明显。用谷氨酸钠盐（ＭＳＧ）损伤雌、雄大鼠，雄性大鼠肾Ｐ４５０４Ａ２水平仍高于正常雄性大鼠肾的，而ＭＳＧ处理的雌性大鼠肾Ｐ４５０４Ａ２水平明显低于正常雌性大鼠的。说明生长激素对雌性大鼠肾Ｐ４５０４Ａ２可能有直接调节作用，而对雄性大鼠肾脏细胞色素Ｐ４５０４Ａ２的影响则较为复杂。
Western blotting was used to detect the effect of growth hormone on the microsomal cytochrome P4504A2 in rat kidney. Rat renal microsomal cytochrome P450 and liver, the same species, gender-specific. Normal male rat renal microsomal cytochrome P4504A2 content than female rats. The content of cytochrome P4504A2 in renal microsomes in male and female rats with hypophysectomy was significantly higher than that in normal control rats, but the difference between male and female rats was not obvious. The level of renal P4504A2 in male rats was still higher than that in normal male rats by glutamate sodium (MSG) injury, whereas the level of renal P4504A2 in MSG-treated female rats was significantly lower than that in normal female rats. The results showed that growth hormone may have a direct regulatory effect on the kidney of rats P4504A2, while the effects on the cytochrome P4504A2 in the kidney of rats are more complicated.