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目的建立大米基质中4种黄曲霉毒素B1、B2、G1和G2的超高效液相色谱荧光检测器的测定方法。方法甲醇水提取大米中的黄曲霉毒素,免疫亲和柱净化富集。C18柱(3.0 mm×50 mm×1.7μm)分离,一定比例的甲醇乙腈水等度洗脱,萤光检测器定量检测。结果黄曲霉毒素B1、G1在0.25μg/L~4.00μg/L线性范围,黄曲霉毒素B2、G2在0.076μg/L~1.20μg/L线性范围,相关系数均在0.999及以上。本方法在大米介质中,总黄曲霉毒素检出限为0.04μg/L。不同加标浓度水平的回收率在75%~96%,精密度在0.79%~4.0%。结论该方法分离度好,分析数据速度快,准确度高,重复性好,适合大米中黄曲霉毒素B1、B2、G1和G2的检测分析。
Objective To establish a method for the determination of four aflatoxins B1, B2, G1 and G2 in rice matrix by ultra performance liquid chromatography with fluorescence detector. Methods Methanol water was used to extract aflatoxins in rice, and the column was purified with immunoaffinity column. C18 column (3.0 mm × 50 mm × 1.7 μm), a certain proportion of methanol acetonitrile water isocratic elution, fluorescent detector quantitative detection. Results The aflatoxins B1 and G1 ranged from 0.25μg / L to 4.00μg / L and the aflatoxins B2 and G2 ranged from 0.076μg / L to 1.20μg / L with the correlation coefficients above 0.999 and above. In this method, the detection limit of total aflatoxins in rice medium is 0.04 μg / L. The recoveries of different spiked levels ranged from 75% to 96% with precision ranging from 0.79% to 4.0%. Conclusion The method has good resolution, fast analysis data, high accuracy and repeatability, and is suitable for the detection and analysis of aflatoxins B1, B2, G1 and G2 in rice.