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据《中华妇产科杂志》1995年30卷第2期报道 上海医科大学妇产科医院丰有吉等,为了解孕激素治疗卵巢癌的作用环节,对甲孕酮在人卵巢上皮性癌细胞增殖中的抑制作用进行了观察。 他们将不同终浓度甲孕酮分别加入人卵巢上皮性癌3AO细胞株培养基内,并设空白对照组培育3日。采用免疫组化及流式细胞间接荧光检测法,测定癌基因C-erbB_2表达程度,并用显微镜观察癌细胞形态变化及集落形成情况。结果显示,空白对照组癌细胞培养后C-erbB_2表达强度无变化,而实验组45％癌细胞C-erbB_2表达强度减弱,癌细胞生长呈抑制状态,细胞数较空白对照组减少10％;药物浓度增至20nmo(?)/L时,可明显抑制癌细胞生长。显微镜观察可见实验组细胞增大、变圆、透亮。空白对照组癌细胞集落遍布于培养皿内,而实验组3
According to the “Chinese Journal of Obstetrics and Gynecology,” 1995 Volume 30, No. 2 reported Shanghai Obstetrics and Gynecology Hospital Feng Youji and so on, in order to understand the role of progesterone in the treatment of ovarian cancer link, progesterone in human ovarian epithelial cancer cell proliferation In the inhibition was observed. They will be different final concentrations of medroxyprogesterone acetate were added to human ovarian epithelial cancer 3AO cell line medium, and set the blank control group for 3 days. Immunohistochemistry and flow cytometry were used to detect the expression of C-erbB_2, and the morphological changes and colony formation of the cancer cells were observed with a microscope. The results showed that there was no change in the expression of C-erbB_2 in the control group, while the expression of C-erbB_2 in 45% of the experimental group was weakened, the growth of the cancer cells was inhibited and the number of cells was reduced by 10% compared with the blank control group. The concentration increased to 20nmo (?) / L, can significantly inhibit the growth of cancer cells. Microscopic examination showed that the experimental group of cells increased, round, bright. The control group of cancer cells colonized in Petri dishes, while experimental group 3