论文部分内容阅读
对收集的22株和GenBank下载的40株葡萄座腔菌Botryosphaeria ITS序列构建了MP(Maximum parsimony)进化树。在以Leucostoma为外群的情况下,来自我国(主要是陕西)的葡萄座腔菌树木溃疡病菌总体上可分为2个大群,BDGroup1与GenBank上报道的B.dothidea被聚在一起,BD Group2菌株全部来自我国关中咸阳,独立成群,与Botryosphaeria属其他种亲缘关系远。培养特征、分生孢子大小和致病性测定显示:来自我国的苹果轮纹病菌、苹果干腐病菌、梨轮纹病菌、桃树流胶病菌同B.dothidea亲缘关系近,在MP树中聚在一起(BD Group1)。ITS序列分析结果支持B.dothidea与B.berengeriana为同物异名的观点。B.ribis与B.parva亲缘关系近,而与B.dothidea亲缘关系远,支持B.ribis与B.dothidea为不同种的观点。
The MP (Maximum parsimony) phylogenetic tree was constructed from 22 collected and 40 Botryosphaeria ITS sequences downloaded from GenBank. In the case of Leucostoma being an outgroup, B. candelnsis canker canker in China (mainly Shaanxi) can be generally divided into two large groups, BDGroup1 and B.dothidea reported in GenBank are clustered together, BD Group2 Strains all come from our country Guanzhong Xianyang, independent groups, and Botryosphaeria belong to other species far. The culture characteristics, conidia size and pathogenicity of apple tree, apple stem rot fungus, pear ring rot fungi and mycoplasma gondii were found to be closely related to B.dothidea in China, Together (BD Group1). ITS sequence analysis supports the view that B.dothidea and B.berengeriana are synonymous. B.ribis is closely related to B.parva and has a close genetic relationship with B.dothidea, supporting B.ribis and B.dothidea as heterogeneous.