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测定了8株分离于自然罹病死亡的椰心叶甲虫体绿僵菌的培养特性并采用其ITS序列进行系统发育分析,结果表明:绿僵菌在以玉米粉作碳源,牛肉膏、蛋白胨作氮源的条件下菌丝的生长速率最快,产孢量最高;绿僵菌对温度和pH的适应范围较广,最适宜的生长温度是26～28℃,pH为6～7时,菌丝生长最快,孢子量最多,绿僵菌的致死温度为55℃.基于8个菌株rDNA的ITS1-5.8S-ITS2区域序列的系统研究结果表明,8个菌株均聚在金龟子绿僵菌小孢变种(Metarhiziumanisopliae var.anisopliae)构成的分支中,明显可分成两群,HY-4、YP-6、SX-2、QH-4为一群,与标准菌株FI1091-AF135214接近,另一群为HK-5、SY-2、SS-2、WC-1,与标准菌株Mb5-EF113340接近.
The phylogenetic analysis of 8 strains of Metarhizium anisopliae isolated from natural deaths and their phylogenetic analysis using ITS sequences showed that Metarhizium anisopliae was used as carbon source, beef extract and peptone as nitrogen source , The mycelial growth rate was the fastest and the sporulation rate was the highest. Metarhizium anisopliae had a wide adaptation to temperature and pH, the most suitable growth temperature was 26 ~ 28 ℃, the mycelial growth was at pH 6 ~ 7 The fastest and the spore quantity was the most, and the lethal temperature of Metarhizium anisopliae was 55 ° C. The systematic study on the sequences of ITS1-5.8S-ITS2 based on 8 strains of rDNA showed that 8 strains were homogeneously clustered in the spore of Metarhizium anisopliae (Metarhiziumanisopliae var. Anisopliae) was divided into two groups, HY-4, YP-6, SX-2 and QH-4 were grouped into a group close to the standard strain FI1091-AF135214 and the other group was HK- SY-2, SS-2, WC-1, close to the standard strain Mb5-EF113340.