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目的:探讨表皮生长因子(Epidermal growth factor,EGF)和口腔鳞癌侵袭转移的关系。方法:采用ELISA和免疫组化法测量不同浓度EGF作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后血管内皮生长因子(vascular endothelial growth factor,VEGF)活性和阳性表达变化;同时采用凝胶电泳迁移实验(EMSA)检测不同浓度EGF下核转录因子-κB(nuclear factor kappa B,NF-κB)的活性变化。结果:不同浓度的EGF(0ng/mL,10ng/mL,40ng/mL,80ng/mL)作用于两种细胞系后,随着外源性EGF浓度的增加,GNM和TSCCa细胞中VEGF活性和阳性表达有增加,NF-κB的活化明显增强,VEGF和NF-κB的活性与EGF有剂量依赖关系,与对照组相比差异有显著性(P<0.05);而在相同的浓度内TSCCa细胞较GNM细胞VEGF和NF-κB的活性增加较明显,两者相比差异有显著性(P<0.05)。结论:EGF可能是通过增加VEGF的活性和阳性表达,调节NF-κB的信号传导路径来促进OSCC细胞侵袭和转移的。
Objective: To investigate the relationship between epidermal growth factor (EGF) and invasion and metastasis of oral squamous cell carcinoma. Methods: The changes of vascular endothelial growth factor (VEGF) activity and the expression of VEGF in oral squamous cell carcinoma TSCCa cell line and cervical lymph node metastatic carcinoma GNM cell line were measured by ELISA and immunohistochemistry. The electrophoretic mobility shift assay (EMSA) was used to detect the changes of nuclear factor kappa B (NF-κB) activity under different concentrations of EGF. Results: After treated with different concentrations of EGF (0 ng / mL, 10 ng / mL, 40 ng / mL, 80 ng / mL), the activity of VEGF in both GNM and TSCCa cells was positive with the increase of exogenous EGF concentration The expression of NF-κB was significantly increased, and the activity of VEGF and NF-κB was in a dose-dependent manner compared with that of the control group (P <0.05). However, in the same concentration of TSCCa cells, The activity of VEGF and NF-κB increased significantly in GNM cells, the difference was significant (P <0.05). Conclusion: EGF may promote the invasion and metastasis of OSCC cells by increasing the activity and expression of VEGF and regulating the signal transduction pathway of NF-κB.