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目的 研究原癌基因c -myc和jun在人胎冠状动脉发育过程中的表达与平滑肌细胞增殖的关系。方法 用原位杂交方法检测 ,胎龄分别为 16周、2 2周 (因治疗需要引产 )的胎儿和意外死亡的足月胎儿冠状动脉前降支c -mycmRNA和junmRNA的表达水平。杂交反应产物用图像分析仪 (MIAS30 0 )作定量分析。结果 C -mycmRNA原位杂交反应产物与被测血管区域面积的百分比在 16周、2 2周和足月胎儿分别是 70、5 6和 10 ;JunmRNA的杂交信反应产物与被测血管区域面积的百分比在这三个时期分别是 6 8、5 3和 8。两个原癌基因在不同阶段的表达均具有显著性差异。结论 本实验首次报道c -myc和jun在人胎冠状动脉发育过程中平滑肌的表达图型 ,c -myc和jun在胎儿冠状动脉平滑肌细胞增殖和内膜的形成过程中可能具有重要的调控作用。
Objective To study the relationship between the expression of protooncogene c -myc and jun in human fetal coronary artery and the proliferation of smooth muscle cells. Methods The in situ hybridization method was used to detect the expression of c-mycmRNA and jun mRNA in the anterior descending coronary artery in fetuses with gestational age of 16 weeks and 22 weeks (due to induction of labor) and unintentional death of full-term fetuses. The hybridization reaction products were quantitatively analyzed using an image analyzer (MIAS30 0). Results The percentage of C-mycmRNA in situ hybridization reaction with the area of the blood vessel examined was 16 weeks, 22 weeks and full-term fetuses were 70, 56 and 10, respectively. The product of JunmRNA’s hybridization reaction and the area of the tested blood vessel The percentages for these three periods are respectively 6 8, 53 and 8. The two proto-oncogenes at different stages of expression were significantly different. Conclusions This study reports for the first time the expression pattern of c-myc and jun in smooth muscle during human coronary artery development. C-myc and jun may play an important regulatory role in fetal coronary artery smooth muscle cell proliferation and intimal formation.