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目的探讨nNOS,iNOS,eNOS的表达与胆管癌临床病理特征及预后的关系。方法检测和比较胆管癌与正常肝外胆管组织的nNOS,iNOS,eNOS表达。结果(1)与正常胆管比较,胆管癌中iNOS及eNOS表达增强(P<0.05),nNOS无表达增强(P>0.05)。(2)iNOS在晚期和中期胆管癌组间阳性表达率差异有显著性(P<0.01);eNOS在晚期、中期、有无远处转移胆管癌组间阳性表达率差异均有显著性(P<0.05)。(3)eNOS阴性表达组预后明显优于阳性表达组(P<0.01),nNOS及iNOS表达与预后无关(P>0.05)。结论(1)胆管癌eNOS的过度表达与胆管癌的临床病理分期、远处转移及预后有关,提示检测胆管癌中eNOS表达可能有助于对胆管癌病情及预后的判断。(2)iNOS表达与胆管癌的临床病理分期关系密切,与有无远处及淋巴结转移、预后无关,推测iNOS可能有促进胆管癌的局部浸润生长,促进病情发展的作用。
Objective To investigate the relationship between the expression of nNOS, iNOS and eNOS and the clinicopathological characteristics and prognosis of cholangiocarcinoma. Methods The expressions of nNOS, iNOS and eNOS in cholangiocarcinoma and normal extrahepatic bile duct tissues were detected and compared. Results (1) Compared with normal bile duct, the expression of iNOS and eNOS in cholangiocarcinoma increased (P <0.05), while the expression of nNOS increased (P> 0.05). (2) There was significant difference of iNOS positive expression rate in advanced cholangiocarcinoma group (P <0.01). The positive expression rate of eNOS in late cholangiocarcinoma group was significantly higher than that in distant metastasis group (P <0.05). (3) The prognosis of eNOS negative expression group was significantly better than that of positive expression group (P <0.01). The expression of nNOS and iNOS had no correlation with prognosis (P> 0.05). Conclusions (1) Overexpression of eNOS in cholangiocarcinoma is associated with clinicopathologic stage, distant metastasis and prognosis of cholangiocarcinoma, suggesting that detecting eNOS expression in cholangiocarcinoma may be helpful to judge the condition and prognosis of cholangiocarcinoma. (2) The expression of iNOS is closely related to the clinicopathological stage of cholangiocarcinoma. It has nothing to do with the distant and lymph node metastasis and prognosis. It is speculated that iNOS may promote the local infiltration and growth of cholangiocarcinoma and promote the progression of the disease.