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为更加精确有效地评估杀菌剂对小麦条锈病的防效,本研究利用real-time PCR检测潜育阶段的分子病情指数(molecular disease index,M DI)并结合田间病情分析,对三种杀菌剂的防效进行评价。春季返青后在品种铭贤169和京0045试验小区的诱发中心接种小麦条锈菌夏孢子,待充分发病后将其铲除。设置播种前2%立克秀拌种,接种后21和28 d分别喷施25%阿米西达和30%苯甲-丙环唑1 500倍液三种处理。发病后调查不同处理的普遍率和严重度,计算病情指数和AUDPC。结果表明,阿米西达和苯甲-丙环唑对小麦条锈病具有良好的防效且能够明显抑制发病中心的扩展,而经立克秀拌种对该病害的防治效果和发病中心扩展的抑制作用均不理想。对京0045于发病中心接种后24和33 d采样,经双重real-time PCR定量检测,计算MDI。结果显示潜育检测获得的M DI与AUDPC之间均具有极显著的相关性;阿米西达和苯甲-丙环唑对潜育期小麦条锈菌的扩展具有明显的持续抑制作用,而立克秀拌种仅对小麦条锈菌潜伏初期的扩展表现出一定的抑制作用。
In order to evaluate the control effect of bactericide on wheat stripe rust more accurately and effectively, this study used real-time PCR to detect the molecular disease index (M DI) during the incubation period and combined with field disease analysis, The effectiveness of the evaluation. Spring back to green in the varieties of Mingxian 169 and Beijing 0045 pilot cell induced inoculation of wheat stripe rust spores, to be fully eradicated after its eradication. Set 2% before planting Li Kexiu seed dressing, 21 and 28 d after inoculation were sprayed with 25% amoxicillin and 30% benzopu-pyrazole 1 500 times the three treatments. After onset, the prevalence and severity of different treatments were investigated and the disease index and AUDPC were calculated. The results showed that amoxicillin and p-tolcyclozole had a good control effect on wheat stripe rust and could obviously inhibit the expansion of the disease center. However, the control effect of the disease and the expansion of the disease center were inhibited The role is not ideal. Beijing 0045 was inoculated 24 and 33 days after the inoculation center, and the MDI was calculated by quantitative double real-time PCR. The results showed that there was a significant correlation between M DI and AUDPC obtained by the latent test. Amoxicillin and benzo-propiconazole significantly inhibited the growth of stripe rust of wheat in the incubation period. Kexiu seed dressing only showed an inhibitory effect on the initial latency of wheat stripe rust.