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目的观察人参皂甙Rh2对小鼠移植瘤生长,肿瘤细胞血管内皮生长因子C(VEGF-C)表达和淋巴管密度的影响,探讨其作用机制。方法用S180瘤株构建55只小鼠移植瘤模型,成瘤后灌服人参皂甙Rh2,观察用药组与对照组移植瘤的生长情况,免疫组织化学染色,比较用药2周、3周后癌细胞VEGF-C的表达及LYVE-1标记的淋巴管密度与对照组的差异。结果接种约第3周开始,对照组移植瘤生长速度明显快于用药组。用药第2周癌细胞VEGF-C表达及淋巴管密度与对照组无差异;第3周VEGF-C表达较对照组弱,淋巴管密度也较对照组低,有差异(P<0.05)。结论人参皂甙Rh2能抑制肿瘤生长,降低淋巴管密度,其机制可能是通过降低VEGF-C在癌细胞的表达,干扰淋巴管的生成。
Objective To observe the effects of ginsenoside Rh2 on the growth of mouse xenografts and the expression of vascular endothelial growth factor C (VEGF-C) and lymphatic vessel density in tumor cells and to explore its mechanism. Methods Fifty-five mice xenografts were established by S180 tumor strain. After the tumor was established, ginsenoside Rh2 was instilled. The growth of xenograft tumors was observed by immunohistochemical staining. The tumor cells were harvested after 2 and 3 weeks of treatment VEGF-C expression and LYVE-1 labeled lymphatic vessel density compared with the control group. Results From the third week after inoculation, the growth rate of the control group was significantly faster than that of the control group. The expression of VEGF-C and the density of lymphatic vessels in cancer cells at the second week of treatment were not different from those in the control group. The expression of VEGF-C in the third week was weaker than the control group, and the density of lymphatic vessels was also lower than that of the control group (P <0.05). Conclusion Ginsenoside Rh2 can inhibit tumor growth and decrease lymphatic vessel density. The mechanism may be that it may interfere with lymphangiogenesis by decreasing the expression of VEGF-C in cancer cells.