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目的:应用低血清培养基在水痘减毒活疫苗生产过程中进行细胞和病毒培养，观察培养效果，验证其对疫苗生产的适用性。方法:采用低血清培养基培养MRC-5细胞和水痘病毒Oka株，以传统培养基的生产为对照，比较细胞生长、病毒感染情况及原液病毒滴度和牛血清白蛋白残留量。采用n t检验对结果进行比较。n 结果:在相同工作细胞库细胞复苏的情况下，低血清培养基与传统培养基培养的细胞和病毒形态，没有明显的差异。低血清培养基与传统培养基培养得到的水痘病毒原液病毒滴度均为5.0～5.1 lg噬斑形成单位/ml，差异无统计学意义(n t＝1.00，n P>0.05)；但低血清培养基生产的水痘病毒原液中牛血清白蛋白残留量(12～19 ng/ml)显著低于传统培养基生产的(39～46 ng/ml)(n t＝8.51，n P0.05). The BSA residue amount of varicella stock with low serum medium (12-19 ng/ml) was significantly lower than that with traditional medium (39-46 ng/ml) (n t=8.51, n P<0.05).n Conclusions:Using low serum medium in the production process of live attenuated varicella vaccine does not affect the virus titer, but significantly lowers the BSA residue, thus can reduce the risk of vaccine adverse reactions caused by bovine origin. Low serum medium improves the quality of vaccine and is suitable for the production of liver attenuated varicella vaccine.