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Patients-derived induced pluripotent stem cells (iPSCs) provide an invaluable tool to study mechanisms of human diseases and also a limitless cellular source for clinical transplantation (Takahashi et al.,2007;Yu et al.,2007;Liu et al.,2011a,2011b;Zhang et al.,2012).Retrovirus- or lentivirus-based delivery systems have been serving as mainstream methods to generate patients-derived iPSCs.However,genomic integrations of reprogramming factors in virally generated iPSCs not only cause insertional mutagenesis but also lead to residual expression of reprogramming factors in iPSCs and their derivatives.