Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Myt

• 作 者: 刘一兵 刘诗文 刘冰莉 秦建光 许通 李孝绪
• 机 构: LiaoningOceanandFisheriesScienceResearchInstitute,CollegeofScienceandEngineering,CollegeofFisheriesa
• 出 处: 海洋湖沼学报(英文)
• 发表日期: 2018年6期
• 关键词: blue MUSSEL MYTILUS galloprovincialis strip SPAWNING SPERM CRYOPRESERVATION
• 基金项目: Supported by the China Scholarship Council and South Australian Research and Development Institute(SARDI).

In this study,a programmable freezing technique has been developed for strip spawned sperm in the blue mussel,Mytilus galloprovincialis.The optimized key parameters include cooling rate,endpoint temperature,thawing temperature,sugar addition and sperm to