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以拔地拉(Saccharum officinarum L.cv.Badila)幼苗为材料,研究了长期(28 d)冷适应处理(白天/夜间:12℃/8℃)对其光合光能利用的影响。结果表明:无论是常温(25℃)还是低温(12℃)测定,冷适应处理明显降低了蔗苗净光合速率(Pn),增加了暗呼吸速率(Rd)和电子传递量子效率和碳同化量子效率比值(ΦPSII/ΦCO2);常温下,冷适应处理降低了不同光强下Fv’/Fm’(和NPQ反向变化)随ΦPSII下降而下降的速率,同时增加了光化学淬灭q P随ΦPSII下降而下降的速率;而低温下,不同光强下Fv’/Fm’及q P随ΦPSII下降而下降的速率与对照没有显著差异;长期冷适应处理通过光化学途径和非光化学途径耗散了光合机构多余激发能,改善了其光合冷敏性,对果蔗抗冷栽培和抗低温育种有重要参考价值。
The effects of long-term (28 d) cold-adapted treatment (day / night: 12 ℃ / 8 ℃) on the photosynthetic light energy utilization of Saccharum officinarum L.cv.Badila seedlings were studied. The results showed that cold acclimation decreased net photosynthetic rate (Pn), increased dark respiration rate (Rd), electron transfer quantum yield, and carbon assimilation quantum yield at both 25 ℃ and 12 ℃, (ΦPSII / ΦCO2). At room temperature, cold acclimation decreased the rate of decrease of Fv ’/ Fm’ (and NPQ reverse) with the decrease of ΦPSII under different light intensities, and at the same time increased the photochemical quenching of qP with ΦPSII The rate of decline of Fv ’/ Fm’ and qp with the decrease of ΦPSII at low temperature did not differ significantly from that of the control; the long-term cold-adapted treatment dissipated the photosynthetic and non-photochemical pathways of photosynthesis Mechanism of excess excitation energy to improve its photosynthetic cold sensitivity, anti-cold cultivation of sugarcane and anti-low temperature breeding has important reference value.