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用酶联免疫方法(DAS、ELISA),对经过热处理和茎尖培养获得的5个品种再生试管苗进行马铃薯X病毒、Y病毒、卷叶病毒的检测。结果5个品系都有3种病毒都脱净的植株,邱北乌芋有6株,邱北白花芋4株,广南白洋芋有9株,广南紫洋芋有7株,马塘紫芋3株。脱毒率最高的品种为邱北白花芋达33.3%,脱毒率最低为广南白洋芋为12.8%。
Five varieties of regenerated plantlets obtained by heat treatment and shoot tip culture were tested for potato X virus, Y virus and leafroll virus by enzyme-linked immunosorbent assay (DAS, ELISA). The results showed that all the 5 strains had three kinds of viruses, including 6 plants of Uighur, 6 plants of Qiu Bei, 4 plants of Guangnan, 9 plants of Guangnan, 7 plants of Maotang, 3 strains. The varieties with the highest detoxification rate were 33.3% of QiuBei white taro and the lowest detoxification rate was 12.8% of Guangnan white taro.