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以巴东木莲叶片为材料,通过对反应体系中Mg2+、dNTP、Taq酶、模板、引物浓度等影响因素的优化,确定了一套适于巴东木莲RAPD分析的稳定反应体系,即25μl的总反应体系中:模板DNA 100 ng,Mg2+2.1 mM,引物0.7μM,dNTP0.20 mM,TaqDNA聚合酶3 U。
Taking the leaves of P. orientalis as materials, a set of stable reaction system suitable for RAPD analysis of M. palmatum was established by optimizing the factors such as Mg2 +, dNTP, Taq enzyme, template, primer concentration in the reaction system, Of total reaction system: template DNA 100 ng, Mg2 + 2.1 mM, primer 0.7 μM, dNTP 0.20 mM, Taq DNA polymerase 3 U.