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以落葵子叶和胚轴为外值体 ,在 MS基本培养基中添加不同浓度激素培养。结果表明 ,胚轴在 NAA0 .1～ 0 .3 mg/ L和 BA1～ 3 mg/ L范围内能产生愈伤组织 ,而子叶在上述培养基上均不能产生愈作组织。胚轴愈伤组织在 NAA0～0 .3 mg/ L 和 BA3 mg/ L 及 KT3 mg/ L 上分化出芽 ,在 1 / 2 MS+ NAA0 .3 mg/ L培养基上 ,生根率和根的质量均最好 ,再生小植侏移栽成活率达 1 0 0 %。
In order to fall off the sunflower cotyledons and hypocotyls outside body, in the MS basic medium to add different concentrations of hormone training. The results showed that the hypocotyls could produce callus in the range of NAA0.1 ~ 0.3 mg / L and BA1 ~ 3 mg / L, while the cotyledons could not produce callus on the above medium. The hypocotyls callus differentiated and budded at NAA0 ~ 0.3 mg / L, BA3 mg / L and KT3 mg / L, and the rooting rate and root mass of the callus at 1/2 MS + NAA0.3 mg / The best survival rate of regenerated planted dwarf shrimp reached 100%.