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长白山区木生担子菌的分离结果表明:被分离的74个菌种巾有148个菌株,分离到62个菌种124个菌株,分离的成功率为85%左右。未被分离成功者多存在于多孔菌,寄主多数为针叶树、倒木、褐色腐朽型。菌种(株)的斜面长势和长速的初步观察,有28个菌种(株)的菌丝生长迅速,粗壮丰满,5—6天能长满斜面;有些种(株)7天以上长满斜面;有30余个菌种(株)13天或更长的时间才能长满斜面;有些只在斜面上稍有生长。同一菌种由于分离的木块、树种等不同,长势和长速差别也很大。菌丝的长势粗壮丰满,生长迅速的菌种,大都属于寄主为阔叶树的白色腐朽型。菌种(株)在灭菌料长势和长速的观测,结果是在斜面上长势好,长速快的菌株,一般在灭菌料上也同样生长良好。菌种(株)在生料上做生长试验,结果只有66—1和76—1两菌种生长良好,它们均属侧耳属,8—9天长透原料,15—20天在原料上长出担子果。通过菌种分离和初步研究说明,所选用的马铃薯—蔗糖—麸皮琼脂培养基是适宜的;像长白山区那样潮湿多雨的地方,担子果吸饱水的情况下,采用的菌木菌丝分离法是一种简单易行的好方法;以此法分离菌种时,立木的成功率最高,伐桩次之,倒木和根基腐朽种类最差;菌丝粗壮,生长快的菌种(株),约占分离菌种(株)的1/4—1/3,它们易于分离到纯菌种;它们大都是腐生于阔叶树,为白色腐朽型,属白腐菌;在分离得到的菌种中能在代料生料上生长的是个别的。
The results showed that 148 isolates from 74 strains were isolated and 124 isolates from 62 strains were isolated. The success rate of isolation was about 85%. Many were not successful in the separation of porous bacteria, the majority of the host conifers, inverted wood, brown decay type. Mycoplasma (strain) of the bevel growth and the initial observation of long-speed, 28 strains of mycelium rapid growth, thick and plump, 5-6 days can be covered with beveled; some species more than 7 days long Full of bevel; more than 30 strains (strains) 13 days or more to be full of bevel; some only slightly grown on the bevel. The same strain due to the separation of wood, tree species, growth and long-term difference is also great. Mycelium growing thick plump, rapid growth of species, mostly belong to the broadleaf host white decay type. Strain (strain) in sterilizing material growth and the observation of long-speed, the result is good growth on the slope, long-speed fast strains, the general sterilization material also grow well. Strains (strains) in the raw material to do the growth test, the results only two strains of 66-1 and 76-1 growth well, they are Pleurotus, 8-9 days long through the raw materials, 15-20 days in raw materials grow Burden fruit. Isolation and preliminary study of the strains indicated that the potato-sucrose-bran agar medium was suitable for selection. In the humid and rainy areas like Changbai Mountain, the hypha mycelium separated Act is a simple and easy way; when this method is used to separate the species, the success rate of standing wood is the highest, followed by stumping, the worst is the inverted wood and the decayed species; , Accounting for about 1 / 4-1 / 3 of the isolated strains (strains), which are easy to isolate pure species; most of them are saprophytic deciduous trees, white decay, belonging to white rot fungi; in isolated strains Can grow on the substitute raw material is individual.