论文部分内容阅读
目的:探究丹酚酸B(Sal B)与甘草次酸(GA)、白藜芦醇(RES)单用及合用对博来霉素诱导肺纤维化小鼠的干预作用。方法:采用气管注射博来霉素(BLM)的方法复制小鼠肺纤维化模型,设置对照组、模型组、醋酸地塞米松组(DXM)组、甘草次酸(GA)组、白藜芦醇(RES)组、丹酚酸B(Sal B)组、GA+RES组、GA+Sal B组、RES+Sal B组、GA+RES+Sal B组,共10组,7 d后开始口服给药治疗,记录小鼠日常情况、体重,于造模后7、14、28 d分别采集小鼠肺部样本,计算肺系数及检测肺组织羟脯氨酸(Hyp)含量。结果:7 d各BLM造模小鼠体重均小于对照组,14 d、28 d各给药组小鼠体重小于对照组且大于模型组;14 d各给药组肺系数明显小于模型组,大于对照组,28 d除RES组外,其余各给药组肺系数均小于模型组;7 d模型组肺组织Hyp含量小于对照组,无显著性差异(P>0.05),14 d、28 d各组Hyp含量不同程度变大,各给药组Hyp含量小于模型组大于对照组,28 d时,DXM组Hyp含量小于模型组且差异有统计学意义(P_(DXM)=0.012 7)。除GA+Sal B组和三药合用组外其余组与DXM组差异有统计学意义。两两合用给药组及三药合用给药组Hyp略小于单用给药组,差异无统计学意义。结论:GA、Sal B、RES单用及合用均对BLM致小鼠肺纤维化显示出一定的干预作用,两两合用给药、三药合用干预效果略好于单味给药,其中两两合用给药组中,Sal B与GA配伍效果相对较好,三味药合用对肺纤维化前期炎症细胞产生阶段影响较大。
Objective: To investigate the effects of Sal B combined with glycyrrhetinic acid (GA) and resveratrol (RES) alone and in combination on the bleomycin-induced pulmonary fibrosis in mice. Methods: Pulmonary fibrosis model was induced by intratracheal instillation of bleomycin (BLM). The control group, model group, DXM group, glycyrrhetinic acid (GA) group, RES group, Sal B group, GA + RES group, GA + Sal B group, RES + Sal B group and GA + RES + Sal B group. The mice were sacrificed on days 7, 14, and 28 after modeling. The lungs of mice were collected and the lung coefficients were calculated. The contents of hydroxyproline (Hyp) in lung tissue were measured. Results: At 7 days, the body weights of mice in BLM model were all less than those in control group. At 14 and 28 days, the body weight of mice in each treatment group was smaller than that in control group and larger than that in model group. On 14th day, the lung coefficient of each model group was significantly smaller than that of model group, In the control group, the pulmonary coefficients of the other groups were all less than those of the model group on the 28th day except for the RES group. The levels of Hyp in the lung tissue on the 7th day in the model group were less than those in the control group (P> 0.05) The content of Hyp in different groups increased to varying degrees, and the Hyp content in each group was smaller than that in the control group. At 28 days, the Hyp content in DXM group was lower than that in the model group (P_ (DXM) = 0.012 7). Except GA + Sal B group and the three drug combination group, the difference between the other groups and DXM group was statistically significant. The two groups of combined administration and three groups of combined administration Hyp slightly less than the single administration group, the difference was not statistically significant. CONCLUSION: Both GA and Sal B, RES alone and in combination show some intervention effects on lung fibrosis induced by BLM in mice. The combined effect of three drugs is better than that of single administration Combined administration group, Sal B and GA compatibility is relatively good, shamisen combination of pre-inflammatory cells in pulmonary fibrosis stage of greater impact.