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纸烟烟雾对肺产生许多有害效应。有人报道纸烟烟雾影响肺中RNA和DNA的组成。本文记述在体外用(14)~C标记的甲醛和乙醛与人肺成纤维细胞孵育的结果。在含有1μCi/ml~(14)C标记的甲醛或1μCi/ml双标记的乙醛的培养液中培养人肺成纤维细胞。细胞与标记的甲醛放在一起10分钟(37℃),然后于37℃孵育60分钟或24小时之后测定放射活性进行计数。将细胞分离为上
Cigarette smoke has many harmful effects on the lungs. It has been reported that cigarette smoke affects the composition of RNA and DNA in the lungs. This article describes the results of in vitro incubation of human lung fibroblasts with (14) ~C labeled formaldehyde and acetaldehyde. Human lung fibroblasts were cultured in a culture medium containing 1 μCi / ml ~ (14) C-labeled formaldehyde or 1 μCi / ml double-labeled acetaldehyde. The cells were counterstained with formaldehyde for 10 minutes (37 ° C) and then measured for radioactivity after incubation at 37 ° C for 60 minutes or 24 hours. The cells are separated as above