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铁氰化钾氧化鲁米诺在碱性介质中产生化学发光,姜黄素对该体系化学发光具有强烈的抑制作用。因此,利用该化学发光的抑制体系,结合反向流动注射技术,建立了测定大黄类药物姜黄素含量的新方法。在优化的条件下,化学发光抑制信号强度ΔI与姜黄素的浓度分别在1×10-7~1×10-6和1×10-6~1×10-5mol/L范围内呈良好的线性关系,检出限为1×10-9mol/L。对2.0×10-6mol/L的姜黄素进行平行测定10次,得相对标准偏差(RSD)为1.8%。方法应用于中药姜黄中姜黄素(总姜黄素计)的含量测定。
Chemiluminescence of luminol oxidized luminol in alkaline medium, curcumin has a strong inhibitory effect on the chemiluminescence of the system. Therefore, this chemiluminescence inhibition system, combined with reverse flow injection technique, was established for the determination of the curcumin content of the new method of rhubarb drugs. Under optimized conditions, the chemiluminescence inhibitory signal intensity ΔI and curcumin concentration showed good linearity in the range of 1 × 10-7 ~ 1 × 10-6 and 1 × 10-6 ~ 1 × 10-5mol / L, respectively Relationship, the detection limit of 1 × 10-9mol / L. Parallel determination of curcumin at 2.0 × 10-6mol / L for 10 times gave a relative standard deviation (RSD) of 1.8%. The method was applied to the determination of curcumin (total curcumin) in traditional Chinese medicine Curcuma longa.