Effect and mechanism of β-L-D4A on DNA polymerase α

• 出 处: World Journal of Gastroenterology
• 发表日期: 2007年46期
• 关键词: DNA nucleoside Polymerase crude analogs toxicity separate purified interferon an

AIM:To investigate the safety of β-L-D4A on DNA polymeraseα. METHODS:Ion exchange chromatography was used to separate DNA polymeraseαfrom crude extract of human Hela cells.Detailed kinetic parameters were determined for β-L-D4A against DNA polymeraseα. RESULTS:DNA polymeraseαwas purified with 4% yield and 31000 units/rag specific activity.The Michaelis constant (Km=3.22μmol/L),50% inhibition concentration (IC50=178.49μmol/L) and inhibition constant (Ki=126μmol/L) of β-L-D4A were determined by kinetic analysis. CONCLUSION:β-L-D4A is a more safe nucleoside for hepatitis B virus (HBV) infection with a lower host toxicity.

AIM: To investigate the safety of β-L-D4A on DNA polymerase α. METHODS: Ion exchange chromatography was used to separate DNA polymerase αfrom crude extract of human Hela cells. Betted kinetic parameters were determined for β-L-D4A against DNA polymeraseα. RESULTS : DNA polymeraseαwas purified with 4% yield and 31000 units / rag specific activity.The Michaelis constant (Km = 3.22μmol / L), 50% inhibition concentration (IC50 = 178.49μmol / L) of β-L-D4A were determined by kinetic analysis. CONCLUSION: β-L-D4A is a more safe nucleoside for hepatitis B virus (HBV) infection with a lower host toxicity.