论文部分内容阅读
目的采用梅毒螺旋体抗体吸收实验(FTA-ABS)和免疫印迹技术(WB)法对临床疑似梅毒患者进行梅毒特异性抗体IgG和IgM检测。探讨该部分患者是否存在既往感染或隐性感染以及有无传染性。方法收集2012年3月-2013年5月于本院就诊的95例疑似梅毒患者血清(TPPA~+/TRUST~-或TPPA~+/TRUST~+滴度≤1∶4,既往无梅毒感染史也无相应的梅毒临床症状或体征,其配偶梅毒血清学结果阴性),采用FTA-ABS和WB法进行IgG和IgM特异性抗体检测。结果 FTA-ABS和WB法检测Ig G阳性病例分别为91例、90例;阳性率分别为95.79%和94.74%。两种方法分别与TPPA法比较,差异均无统计学意义(P>0.05)。FTA-ABS和WB法检测IgM阳性病例分别为16例和18例;两者检出阳性率分别为16.84%和18.95%。两种方法阳性检出率差异均无统计学意义(P>0.05)。结论 FTA-ABS和WB法IgG检测结果与TPPA符合率分别为95.79%和94.74%,证实疑似梅毒患者既往存在隐性梅毒感染史;在TPPA~+/TRUST~-或TPPA~+/TRUST~+滴度≤1∶4患者中,FTA-ABS和WB法IgM阳性检测结果分别为16例、18例,提示仍有少数患者可能存在近期感染。
Objective To detect syphilis-specific IgG and IgM in patients with clinically suspected syphilis using the Treponema pallidum antibody Absorption Assay (FTA-ABS) and Western Blot (WB). This part of the patients to explore whether there is a past infection or latent infection and whether contagious. Methods Serum (TPPA ~ + / TRUST ~ - or TPPA ~ + / TRUST ~ + titers ≤1: 4) were collected from 95 patients with suspected syphilis in our hospital from March 2012 to May 2013. No previous history of syphilis infection There was no corresponding clinical symptoms or signs of syphilis, and his spouse’s syphilis serology was negative. The specific antibodies against IgG and IgM were detected by FTA-ABS and WB methods. Results The positive rates of Ig G in FTA-ABS and WB assays were 91 and 90, respectively. The positive rates were 95.79% and 94.74% respectively. The two methods were compared with the TPPA method, the difference was not statistically significant (P> 0.05). IgM positive cases detected by FTA-ABS and WB method were 16 cases and 18 cases, respectively. The positive rates of IgA and IgA were 16.84% and 18.95% respectively. There was no significant difference between the two methods (P> 0.05). Conclusion The coincidence rates of FTA-ABS and WB IgG test and TPPA were 95.79% and 94.74%, respectively, confirming the previous history of latent syphilis infection in patients with suspected syphilis. In TPPA ~ + / TRUST ~ - or TPPA ~ + / TRUST ~ Titers ≤ 1: 4 patients, FTA-ABS and WB IgM positive test results were 16 cases, 18 cases, suggesting that there are still few patients may have recent infections.