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目的探讨环氧合酶-2的表达与子宫肌瘤发病机制之间的相关性,研究其医学价值,为临床提供借鉴意义。方法选取2011年6月—2012年6月收治的施行子宫肌瘤切除术的患者30例,将其子宫肌瘤组织作为观察组,另择取其邻近的正常平滑肌组织患者,将其作为对照组。采用免疫组化的检测方式对环氧合酶-2进行检测,同时运用蛋白印迹法检测蛋白水平中的环氧合酶-2的表达。收集两组患者的临床资料,对其结果进行回顾性分析。结果通过免疫组化检测中发现两组患者中均有环氧合酶-2的表达,对照组13.33%(4/30)的细胞阳性指数明显低于观察组46.67%(14/30),两组比较差异具有统计学意义(χ2=7.935,P<0.05)。蛋白印迹法检测发现对照组的环氧合酶-2的表达0.199±0.054比观察组0.871±0.033低,两组比较差异有统计学意义(t=58.161,P<0.05)。结论环氧合酶-2的表达对子宫肌瘤发病机制有一定程度的作用。
Objective To investigate the correlation between the expression of cyclooxygenase-2 and the pathogenesis of uterine leiomyoma, to study its medical value and provide reference for clinical application. Methods Thirty patients with myomectomy who underwent myomectomy between June 2011 and June 2012 were enrolled in this study. The uterine fibroids were selected as the observation group and the adjacent normal smooth muscle tissues were selected as the control group . Cyclooxygenase-2 was detected by immunohistochemistry and protein expression of cyclooxygenase-2 was detected by Western blotting. The clinical data of two groups were collected and the results were retrospectively analyzed. Results The expression of cyclooxygenase-2 in both groups was found by immunohistochemistry. The positive index of 13.33% (4/30) in control group was significantly lower than 46.67% (14/30) in observation group The differences between the two groups were statistically significant (χ2 = 7.935, P <0.05). Western blotting showed that the expression of cyclooxygenase-2 in the control group was 0.199 ± 0.054 lower than that of the observation group (0.871 ± 0.033), the difference was statistically significant (t = 58.161, P <0.05). Conclusion The expression of cyclooxygenase-2 has a certain degree of effect on the pathogenesis of uterine fibroids.