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目的研究低浓度肠安Ⅰ号制作的安慰剂对番泻叶灌胃联合传统束缚应激诱导腹泻型肠易激综合征(IBS)大鼠模型的影响。方法采用番泻叶灌胃联合传统束缚应激诱导腹泻型IBS大鼠模型,将实验动物分为8组:正常组、模型组、肠安Ⅰ号高、中、低剂量组、含有低浓度(5%、10%)肠安Ⅰ号的安慰剂两组和阳性药对照组。观察各组大鼠在20、40、60、80mmHg下行直肠扩张,采用腹壁撤退反射(AWR)评分测定各组大鼠在20、40、60、80mmHg直结肠扩张压力下疼痛反应,观察本安慰剂对IBS大鼠内脏高敏感的影响。结果番泻叶灌胃联合传统束缚应激诱导腹泻型IBS大鼠模型在造模后第1天各实验组和用药结束后模型组明显增高(P<0.05),显示造模成功。中药对照药肠安Ⅰ号能降低IBS大鼠内脏敏感性的AWR评分(P<0.05,P<0.01),且有一定的量效关系。安慰剂组不能降低其AWR评分(P>0.05)。结论肠安Ⅰ号高、中、低剂量组对番泻叶灌胃+传统束缚应激诱导腹泻型IBS大鼠的内脏高敏感性有一定的调节作用,含有低浓度肠安Ⅰ号制作的安慰剂对IBS大鼠的内脏高敏感则没有调节作用。
Objective To study the effect of placebo in low-concentration Chang’an No.Ⅰ on the rat model of diarrhea-predominant irritable bowel syndrome (IBS) induced by sennae infusion combined with traditional restraint stress. Methods The experimental animals were divided into 8 groups: normal group, model group, Chang’an No.1 high dose, middle dose and low dose group, 5%, 10%) of the placebo two intestines and positive control group. The rats in each group were observed for rectal distension at 20, 40, 60 and 80 mmHg, and the pain response was measured by AWR score at 20, 40, 60 and 80 mmHg. The placebo On the visceral hypersensitivity in IBS rats. Results The experimental group and the model group at the end of the first day after model administration were significantly increased (P <0.05). The results showed that the model was successfully established. The traditional Chinese medicine Chang’an I could reduce the visceral sensitivity of IBS rats AWR score (P <0.05, P <0.01), and have a certain dose-effect relationship. Placebo did not reduce its AWR score (P> 0.05). Conclusion The high, medium and low dose of Chang’an No.1 group can regulate the visceral hypersensitivity of diarrhea-predominant IBS rats by intragastric administration of senna and traditional restraint stress, and it has the effect of constipation Agents on IBS rat visceral hypersensitivity is not a regulatory role.