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目的 探讨大鼠烟雾吸入后透明质酸增多与大鼠肺吸入性损伤的关系。方法 ４０ 只ＳＤ 大鼠随机分成５ 组：正常对照组和烟雾吸入后不同时间观察组。分别测定血清、支气管肺泡灌洗液和肺组织中的透明质酸含量，支气管肺泡灌洗液中的炎性细胞比例及蛋白含量。结果 烟雾吸入后，血清和肺组织中的透明质酸含量升高，１２ ｈ 达到高峰［ 血清：１２ ｈ 组（４７０．４±９７ ．５） μｇ／Ｌ，对照组（１８６．４ ±３１．５） μｇ／Ｌ；肺组织：１２ ｈ 组（６９７ ．５±１１５．１）μｇ／Ｌ，对照组（２０２ ．６ ±３９．４） μｇ／Ｌ］ 。而支气管肺泡灌洗液的各时间观察组升高不显著。支气管肺泡灌洗液蛋白含量６ ｈ 达高峰；细胞数在１２ ｈ 最多，主要是中性粒细胞。结论 烟雾吸入后支气管内炎性细胞以中性粒细胞为主。肺内透明质酸含量高于血液。透明质酸似可作为烟雾吸入性肺损伤的病理指征。
Objective To investigate the relationship between the increase of hyaluronic acid and the inhalation injury of lung in rats after smoke inhalation. Methods Forty Sprague-Dawley rats were randomly divided into five groups: normal control group and observation group at different times after smoke inhalation. The content of hyaluronic acid in serum, bronchoalveolar lavage fluid and lung tissue, the proportion of inflammatory cells in bronchoalveolar lavage fluid and protein content were measured respectively. Results The levels of hyaluronic acid in serum and lung tissue increased after inhalation of smoke, reaching the peak at 12 h [serum: 470.4 ± 97.5 μg / L for 12 h and 186.4 ± 31 for control group. 5) μg / L; lung tissue: 12 h (697.5 ± 115.1) μg / L and control group (202.6 ± 39.4) μg / L]. The bronchoalveolar lavage fluid in each observation group did not increase significantly. The protein content of bronchoalveolar lavage fluid peaked at 6 h; the number of cells reached the maximum at 12 h, mainly neutrophils. Conclusion After bronchial inhalation, the inflammatory cells in bronchus are mainly neutrophils. Hyaluronic acid content in the lungs is higher than blood. Hyaluronic acid can be used as a pathological indicator of smoke inhalation lung injury.