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目的:探讨间充质干细胞(MSCs)对趋化因子VEGF的定向迁移能力与其分化状态之间的关系。方法:本实验运用采用Percoll分离法在体外培养并扩增大鼠骨髓来源MSCs,应用抗氧化剂诱导方案诱导MSCs向神经样细胞分化,运用Boyden chamber及Dunn chamber趋化性迁移装置研究了在趋化因子VEGF诱导下不同分化状态的间充质干细胞定向迁移,比较了各分化状态下细胞的迁移速度和迁移效率。结果:Boyden chamber实验结果显示下室加入不同浓度VEGF后,不同状态细胞向同一浓度VEGF迁移的数量不同,不同浓度VEGF诱导同一状态细胞的迁移数量也不同;Dunn chamber的实验结果显示在某一分化阶段(预诱导24小时)的MSCs具有更高的迁移效率。结论:MSCs的分化影响了其向VEGF的定向迁移,也就是说,不同分化状态的MSCs显示出不同的迁移行为。
Objective: To investigate the relationship between the ability of mesenchymal stem cells (MSCs) to direct the migration of chemokine VEGF and its differentiation status. Methods: Percoll isolation method was used to culture and amplify rat bone marrow-derived MSCs in vitro. Antioxidant-induced MSCs were induced to differentiate into neuron-like cells. Boyden chamber and Dunn chamber chemotactic migration apparatus were used to study the effect of chemotaxis The directional differentiation of mesenchymal stem cells with different differentiation status induced by VEGF was compared. The migration speed and migration efficiency of cells under different differentiation status were compared. Results: The results of Boyden chamber showed that the number of cells migrated from different states to the same concentration of VEGF varied with the concentration of VEGF in the lower chamber. The migration of cells in the same state with different concentrations of VEGF was also different. Dunn chamber’s results showed that in a certain differentiation Stage (pre-induction 24 hours) MSCs have higher migration efficiency. Conclusion: The differentiation of MSCs affects its directional migration to VEGF. That is to say, MSCs with different differentiation states show different migration behaviors.