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目的 :为获得 SEO型病毒基因组更为详尽的资料 ,也为局部爆发的原因提供科学的资料。方法 :病毒分离、Mc Abs分型 RT- PCR扩增及核苷酸序列测定。结果 :首次测出我国 SEO型病毒较为完整的全 S片段及部分 L 片段和 M片段并与 76 - 118、Seoul、SR1 1 (在全 S片段 ) ;76 - 118、C4、L99、Seoul(在 L 片段 ) ;10 A、A9、C1 - 1 、JB3、L99、SD2 2 7、SD70 、SEO(M片段 )株核苷酸同源性进行比较 ,分别为 6 4.2 %、 91.89%、 96 .8%及 70 .8%、 72 .0 %、 95 .3%、 95 .9%及 97.3%、6 3.0 %、 6 6 .0 %、 93.0 %、 94.3%、 96 .3%、 97.3%、 94.7推导出的氨基酸同源性分别为 :80 .5 %、 95 .4%、 97.36 %及 75 .6 %、75 .1%、 97.7%、 97.36 %及 96 .0 %、 78.8%、 79.8%、 94.9%、 98.0 %、 97.0 %、 98.0、 97.0 %。结论 :1不存在病毒基因组间的重排 ,即基因重排不是造成此次 HFRS流行的原因。 2 SEO型病毒表现出明显的地理聚集现象。 3选用与本地区流行的汉坦病毒型别相同的疫苗是行之有效的防止措施
Objectives: To provide more detailed information on the SEO virus genome and to provide scientific information on the causes of local outbreaks. Methods: Virus isolation, Mc Abs typing RT-PCR amplification and nucleotide sequencing. Results: The complete S segment, partial L segment and M segment of SEO virus were detected for the first time in China with 76-118, Seoul, SR1 1 (in whole S segment), 76-118, C4, L99, Seoul L fragment); nucleotide homology of 10 A, A9, C1 - 1, JB3, L99, SD2 2 7, SD70 and SEO (M fragment) were compared, which were 6.22%, 91.89%, 96.8 % And 70.8%, 72.0%, 95.3%, 95.9% and 97.3%, 6 3.0%, 6.6%, 93.0%, 94.3%, 96.3%, 97.3%, 94.7% The deduced amino acid homologies were 80.5%, 95.4%, 97.36%, 75.6%, 75.1%, 97.7%, 97.36% and 96.0%, 78.8%, 79.8% 94.9%, 98.0%, 97.0%, 98.0, 97.0%. Conclusions: 1 There is no rearrangement between viral genomes, ie, gene rearrangement is not responsible for the epidemic of HFRS. 2 SEO virus showed obvious geographical aggregation phenomenon. 3 The use of the same type of vaccine as the popular Hantaan virus in the region is an effective preventive measure