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前言大豆花叶病毒(Soybean mosaic virus,SMV)是大豆上最普遍的病害之一,严重影响大豆的产量和品质,通过筛选与病毒互作的寄主蛋白,根据互作蛋白的功能和两者的作用机制可揭示病毒侵染的分子机制。对于病毒编码的膜蛋白或者膜相关特性的蛋白,由于具有膜结合特性,在生物体内的互作多发生在膜上。传统的酵母双杂交系统发生在核内,试剂盒的载体上加了核定位信号
INTRODUCTION Soybean mosaic virus (SMV) is one of the most common diseases in soybean, which seriously affects the yield and quality of soybean. By screening host proteins that interact with viruses, Mechanism of action reveals the molecular mechanism of viral infection. For virally encoded membrane proteins or membrane-associated proteins, interactions within the organism occur more frequently on membranes due to membrane-bound properties. The traditional yeast two-hybrid system occurs in the nucleus, with a nuclear localization signal added to the vector of the kit