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超氧化物岐化酶(SOD)由于具有消除氧自由基的功能,在医药、保健品中具有重要应用价值。我国目前SOD产品主要是从牲畜血液中提取,这个方法所用原料有限,SOD质量不稳定。80年代后,美国和日本先后开发了用发酵法生产SOD,大大地降低了生产成本。但由于SOD为胞内蛋白,因而发酵法产生SOD后提取工艺极为复杂,至少需要六步,一般经过细胞破碎、离心、盐析、透析、离子交换层析(2～3次)和凝胶层析等才能达到比活>3000u/mg。由于细胞破碎主要为机械法(如球磨和超声波法),胞内所有蛋白都释放出来,SOD比活只有10～30u/mg,因而后续SOD纯化
Superoxide dismutase (SOD) has the important application value in medicine and health care products due to the function of eliminating oxygen free radicals. At present, our country SOD products are mainly extracted from the blood of livestock, this method uses limited raw materials, SOD quality is not stable. After the 1980s, the United States and Japan have developed the use of fermentation production of SOD, greatly reducing production costs. However, since SOD is an intracellular protein, the extraction process of SOD produced by the fermentation method is extremely complex and requires at least six steps. Generally, cells are subjected to cell disruption, centrifugation, salting out, dialysis, ion exchange chromatography (2-3 times) and gel layer Analysis and so on to achieve specific activity> 3000u / mg. Since cell disruption is mainly mechanical (such as ball milling and ultrasonic method), all intracellular proteins are released, SOD only 10 ~ 30u / mg specific activity, and subsequent purification of SOD