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应用分子生物学ＰＣＲ－ＳＳＣＰ法对正常足月新鲜胎盘３０例，葡萄胎（ＨＭ）终止后发展成持续性滋养细胞病（ＰＴＤ）３０例，未发展成持续性滋养细胞病（非ＰＴＤ）３０例，恶性滋养细胞疾病（ＧＴＤ）３０例Ｃ－Ｈａ－ｒａｓ基因点突变进行检测。结果：正常足月胎盘无Ｃ－Ｈａ－ｒａｓ基因点突变；在恶性ＧＴＤ中Ｃ－Ｈａ－ｒａｓ基因突变的阳性率３６．７％，ＰＴＤ中阳性率４３．３％，非ＰＴＤ中阳性率１６．７％。提示：Ｃ－Ｈａ－ｒａｓ基因点突变与ＧＴＤ的发生、发展和恶性演变有关；阳性率随组织学分级差而升高，反映恶性ＧＴＤ的恶性程度。
30 cases of normal full-term fresh placenta were developed by molecular PCR-SSCP, and 30 cases of persistent trophoblastic disease (PTD) developed after the termination of hydatidiform mole (HM). They did not develop persistent trophoblastic disease (non-PTD) 30 Cases, 30 cases of malignant trophoblastic disease (GTD) C-Ha-ras gene mutations were detected. Results: There was no C-Ha-ras gene mutation in normal term placenta. The positive rate of C-Ha-ras gene mutation in malignant GTD was 36.7%, the positive rate in PTD was 43.3% and the positive rate in non-PTD was 16 .7%. It is suggested that the point mutation of C-Ha-ras gene is related to the occurrence, development and malignant transformation of GTD. The positive rate increases with the difference of histological grade, which reflects the malignant degree of malignant GTD.