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[目的 ]比较青蒿琥酯敏感株与抗性株恶性疟原虫对咯萘啶、甲氟喹和奎宁的敏感性 ;了解这 3种药物与青蒿琥酯伍用对抗青蒿琥酯恶性疟原虫的体外抗疟作用。 [方法 ]应用Rieckmann体外微量法测定敏感株与抗性株原虫对上述 4种药物的敏感性 ,同时测定青蒿琥酯分别与咯萘啶、甲氟喹、奎宁伍用对抗性株原虫的敏感性。 [结果 ]咯萘啶、甲氟喹、奎宁和青蒿琥酯对敏感株的ID5 0 分别为 5 9 0、 6 9 7、 2 83 8和 9 6nmo1/L ;对抗性株的ID5 0 依次为 170 6、 6 3 2、 2 72 4及 85 1nmo1/L。咯萘啶与青蒿琥酯伍用 ,其ID5 0 分别为两药单用组的 1/4 7(36 6 / 170 6 )和 1/ 3 7(2 2 8/ 85 1)。甲氟喹与青蒿琥酯伍用中 ,其ID95 分别为两药单用组的 1/ 4(40 / 16 0 )和 1/ 12 5 (3 2 / 40 0 )。奎宁与青蒿琥酯伍用 ,其ID95 分别为单用组的 1/ 16 (80 / 12 80 )和 1/ 12 5 (3 2 / 40 0 )。 [结论 ]抗青蒿琥酯恶性疟原虫对甲氟喹和奎宁无交叉抗性 ,这两种药物分别与青蒿琥酯伍用 ,对抗青蒿琥酯恶性疟原虫有较明显增效作用。
[Objective] To compare the susceptibility of artesunate-sensitive and resistant strains of Plasmodium falciparum to pyronaridine, mefloquine and quinine; to understand the three drugs against artesunate with artesunate Malaria parasites in vitro anti-malarial effect. [Method] The susceptibility of sensitive strains and resistant protozoa to the above four drugs was determined by Rieckmann in vitro microdilution method. The sensitivity of artesunate to pyronaridine, mefloquine, Sensitivity. [Result] The ID50 values of pyronaridine, mefloquine, quinine and artesunate against the susceptible strains were 590, 697, 2883 and 96nmo1 / L, respectively. The ID50 of the resistant strains was in turn 170 6, 6 3 2, 2 72 4 and 85 1 nmo 1 / L. Pyronaridine was used in combination with artesunate with ID50 values of 1/4 7 (36 6/170 6) and 1 3/7 (2 2 8/85 1), respectively. In the combination of mefloquine and artesunate, ID95 was 1/4 (40/160) and 1/125 (3 2/40 0) respectively. Quinine was used in combination with artesunate with ID95 of 1/16 (80/1280) and 1/12 5 (3/2/40 0), respectively. [Conclusion] There was no cross-resistance between Plasmodium falciparum and mefloquine against quinquefacil artesunate. These two drugs were respectively used in combination with artesunate, and had obvious synergistic effect against Artesunate falciparum .