麝香糖蛋白成分对白三烯B_4激活的大鼠中性白细胞功能的影响

• 作 者: 王文杰 白金叶 程桂芳 朱秀媛
• 机 构: 中国医学科学院中国协和医科大学药物研究所
• 出 处: 中国中药杂志
• 发表日期: 1998年04期
• 关键词: 中性白细胞 B_4 大鼠 超氧阴离子 葡糖醛酸 白三烯 溶酶体酶 β-葡糖苷酸酶 作用机制 细胞色素

为观察麝香糖蛋白成分麝香１对体外白三烯Ｂ４激活的大鼠中性白细胞某些功能，包括超氧阴离子产生和溶酶体酶释放的影响，采用大鼠中性白细胞体外温孵系统，用细胞色素Ｃ还原法测定超氧阴离子的产生量。以酚酞葡糖醛酸和ＭｉｃｒｏｃｏｃｕｓＬｙｓｏｄｅｉｋｔｉｃｕｓ为基质的酶反应法测定β葡糖苷酸酶和溶菌酶的释放量。结果：与对照组比较，麝香１在１μｇ／ｍｌ～１００μｇ／ｍｌ终浓度下，可使中性白细胞超氧阴离子生成量增加２８７％～２０２１％，可使β葡糖苷酸酶释放量降低３％～４６％，使溶菌酶释放量降低６％～３２％。提示麝香１对ＬＴＢ４激活的大鼠中性白细胞的功能有明显影响。抑制溶酶体酶释放可能是麝香抗炎作用机制之一

To observe the effects of rutin-1, a rutin component of protein, on rat leukotriene-activated rat neutrophils, including the production of superoxide anion and release of lysosomal enzymes, an in vitro incubation system for rat neutrophils was used. The cytochrome C reduction method was used to measure the production of superoxide anion. Enzymatic reaction method based on phenolphthalein glucuronic acid and Micrococus Lysodexticus was used to determine the release of βglucuronidase and lysozyme. Results:Compared with the control group, Huxiang-1 increased the production of superoxide anion in neutrophils by 287%～2021% at a final concentration of 1μg/ml~100μg/ml, which could make βglucuronide The enzyme release was reduced by 3% to 46%, and the lysozyme release was reduced by 6% to 32%. It is suggested that Ru Hong-1 has a significant effect on the function of LTB4-activated rat neutrophils. Inhibition of lysosomal enzyme release may be one of the anti-inflammatory mechanisms of Musk