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目的:建立经真菌转化前后三七中人参皂苷Rg3的含量测定方法。方法:采用HPLC法,色谱柱为Phenomex C18(250mm×4.6mm,5μm),流动相为乙腈-水,梯度洗脱;柱温30℃,检测波长为203nm,流速1.0mL/min,进样量为20μL。结果:人参皂苷Rg3的回归方程:Y=35672013X-2413,r=0.99992,在0.0019～0.2488 mg/mL范围内有良好的线性关系,平均回收率为99.56%,RSD=1.48%(n=8)。结论:该方法简便、快捷、准确、重复性好,可用于三七发酵产物中人参皂苷Rg3的含量测定。
Objective: To establish a method for the determination of ginsenoside Rg3 in Panax notoginseng through transformation of fungi. Methods: The HPLC method was as follows: Phenomex C18 (250mm × 4.6mm, 5μm) was used as the mobile phase. The mobile phase consisted of acetonitrile and water. The column temperature was 30 ℃, the detection wavelength was 203nm and the flow rate was 1.0mL / 20μL. Results: The regression equation of ginsenoside Rg3 was Y = 35672013X-2413, r = 0.99992, with a good linear relationship in the range of 0.0019 ~ 0.2488 mg / mL with the average recovery of 99.56% and RSD of 1.48% (n = 8) . Conclusion: The method is simple, rapid, accurate, reproducible and can be used for the determination of ginsenoside Rg3 in the fermentation product of Panax notoginseng.