论文部分内容阅读
目的：探索肿瘤抑制基因ｐ５３、ｐ１６变异在脑肿瘤发病中的意义。方法：对２３例脑肿瘤病人原发肿瘤标本用免疫组化ＬＳＡＢ法行肿瘤抑制基因ｐ５３、ｐ１６蛋白检测。结果：星形细胞瘤Ⅰ级９例，ｐ１６蛋白４例表达３０％以下，５例不表达，ｐ５３蛋白３例表达３０％以下，６例不表达；Ⅱ～Ⅳ级７例，ｐ１６蛋白１例表达３０％以下，６例不表达，ｐ５３蛋白４例表达３０％～６０％，３例不表达；脑膜瘤、垂体腺瘤７例，ｐ１６蛋白３例表达３０％以下，４例不表达，ｐ５３蛋白２例表达３０％以下，５例不表达；结论：ｐ５３、ｐ１６蛋白缺失、变异是肿瘤发生的重要原因之一，ｐ５３变异主要见于恶性胶质细胞瘤，偏良性的胶质细胞瘤和脑膜瘤、垂体瘤，ｐ５３蛋白轻度阳性表达，提示肿瘤可能处于早期恶性变过程中。ｐ１６蛋白缺失多见于恶性胶质瘤，也见于部分良性肿瘤。
Objective: To explore the significance of the mutation of tumor suppressor gene p53 and p16 in the pathogenesis of brain tumor. Methods: Tumor suppressor genes p53 and p16 were detected by immunohistochemical LSAB method in 23 cases of primary brain tumor patients. Results: Astrocytoma was grade Ⅰ in 9 cases, p16 protein in 4 cases was less than 30%, 5 cases was not expressed, p53 protein was less than 30% in 3 cases, 6 cases were not expressed; Ⅱ ~ Ⅳ grade in 7 cases, p16 protein in 1 case The expression of p53 protein was 30% or less in 6 cases, 30% -60% in 4 cases, and 3 cases were not expressed in 3 cases. Mesentery, pituitary adenoma in 7 cases, p16 protein in 3 cases were less than 30%, 4 cases did not express p53 2 cases expressed less than 30% protein and 5 cases did not express. Conclusion: The deletion and mutation of p53 and p16 proteins are one of the most important causes of tumorigenesis. P53 mutation mainly occurs in glioblastoma, benign glioma and meninges Tumors, pituitary tumors, p53 protein mild positive expression, suggesting that the tumor may be in the process of early malignant transformation. Deletion of p16 protein is more common in malignant gliomas, also found in some benign tumors.