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采用测定肺泡巨噬细胞(AM)趋化性和AM产生中性粒细胞趋化因子的实验技术。观察到糖皮质激素体外直接作用于大鼠AM悬液或大鼠皮下注射7d都可抑制AM的趋化性,并且肺灌洗液中的AM数量明显减少;但注射3d组无上述效应。以糖皮质激素处理AM 16h可使AM产生的中性粒细胞趋化因子强度明显降低。结果表明糖皮质激素可抑制AM的趋化性和抑制AM产生中性粒细胞趋化因子。提示这可能是较长期应用糖皮质激素抑制免疫和炎症反应、降低肺部防御能力的部分机理。
Experimental techniques were used to determine the chemotaxis of alveolar macrophages (AM) and the production of neutrophil chemokines by AMs. It was observed that the direct effect of glucocorticoid on rat AM suspension or subcutaneous injection of rat for 7 days inhibited the chemotaxis of AM and the number of AM in lung lavage fluid was significantly reduced. However, the above effects were not observed in the 3d group. AM 16h treatment with glucocorticoids significantly reduced the intensity of neutrophil chemokines produced by AM. The results indicate that glucocorticoids can inhibit the chemotaxis of AM and inhibit the production of neutrophil chemokines by AM. Suggesting that this may be part of the longer-term use of glucocorticoids to suppress immune and inflammatory responses and reduce lung defenses.