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目的:观察贞芪扶正胶囊对再生障碍性贫血(AA)模型大鼠重组人红细胞生成素(EPO),CD34~+细胞,白细胞介素-2(IL-2),白细胞介素-11(IL~(-1)1)的影响,探讨其相关的作用机制。方法:按随机数字将60只清洁级Wistar大鼠分为6组,分别为正常组、模型组、贞芪扶正胶囊低、中、高剂量组(5,10,20 g·kg~(-1))及阳性药组(司坦唑醇混悬液,0.004 g·kg~(-1)),除正常组外,其余均釆用5-氟尿嘧啶(5-FU)与马利兰联合建立大鼠AA模型。模型成功后,给药组给予相应药物给药,阳性药组给予司坦唑醇混悬液,正常组与模型组给予相同体积的生理盐水,连续ig 30 d,以外周血细胞计数,骨髓单个核细胞(BMNC)计数,酶联免疫吸附测定(ELISA)法检测IL-2,IL~(-1)1,EPO,肿瘤坏死因子-α(TNF-α)水平,CD34~+抗原和Fas抗原检测为主要观察指标综合评价贞芪扶正胶囊的干预效果。结果:与正常组比较,模型组的外周血白细胞(WBC),血小板(PLT),红细胞(RBC)及血红蛋白(Hb),BMNC,IL~(-1)1,CD3~+T细胞比例,CD3~+CD4~+T细胞比例,CD34~+抗原荧光量均明显降低,IL-2,EPO,TNF-α,Fas抗原荧光量明显升高(P<0.01);与模型组比较,司坦唑醇组、贞芪扶正胶囊高剂量组的WBC,RBC,PLT,Hb,BMNC均有所升高(P<0.05,P<0.01),贞芪扶正胶囊中、高剂量组的IL~(-1)1,CD3~+T细胞,CD3~+CD4~+T细胞比例,CD34~+抗原荧光量升高,IL-2,EPO,TNF-α,Fas抗原荧光量降低降低(P<0.05,P<0.01);与司坦唑醇组比较,贞芪扶正胶囊中、高剂量组的WBC,CD3~+T细胞比例,CD3~+CD4~+T细胞比例,IL~(-1)1,CD34~+抗原荧光量较高(P<0.05),IL-2,TNF-α,Fas抗原荧光量较低(P<0.05)。结论:贞芪扶正胶囊能够改善AA大鼠外周血细胞状况、骨髓造血组织功能的恢复,增强免疫功能,可能与调控因子EPO,IL-2,IL~(-1)1水平和CD34~+细胞密切相关。
Objective: To observe the effects of Zhenqi Fuzheng Capsule on the expression of recombinant human erythropoietin (EPO), CD34 + cells, interleukin-2 (IL-2), interleukin-11 ~ (-1) 1) to explore its related mechanism of action. Methods: Sixty clean Wistar rats were divided into six groups according to random numbers: normal group, model group, Zhenqi Fuzheng capsule low, medium and high dose groups (5, 10, 20 g · kg -1 ) And positive control group (stanozolol suspension, 0.004 g · kg -1). All rats in the control group were treated with 5-fluorouracil (5-FU) model. After the model was successfully administered, the drug was given to the administration group, the positive drug group was given the suspension of stanozolol, the normal group and the model group were given the same volume of saline, continuous ig 30 d, peripheral blood cell count, bone marrow single nucleus The levels of IL-2, IL-1, EPO and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA) As the main index comprehensive evaluation of the effect of Zhenqi Fuzheng capsule intervention. Results: Compared with the normal group, the ratio of WBC, PLT, RBC, Hb, BMNC, IL-1, CD3 + T cells in the model group, (P <0.01). Compared with the model group, the ratio of ~ (+) CD4 ~ + T cells and CD34 ~ + antigen fluorescence decreased significantly (P <0.05, P <0.01). The levels of IL-1 and IL-1 in Zhenqi Fuzheng Capsule high-dose group were significantly higher than that in Zhenqi Fuzheng Capsule high- ) 1, the proportion of CD3 ~ + CD4 ~ + T cells and the amount of CD34 ~ + antigen in CD3 ~ + T cells increased, while the levels of IL-2, EPO, TNF- <0.01). Compared with stanozolol group, the ratio of CD3 + T cells, CD3 + CD4 + T cells, IL-1, CD34 The fluorescence intensity of ~ + antigen was higher (P <0.05), and the fluorescence of IL-2, TNF-α and Fas antigen was lower (P <0.05). Conclusion: Zhenqi Fuzheng Capsule can improve peripheral blood cell status, restore the function of bone marrow hematopoietic tissue and enhance immune function in AA rats, which may be related with the regulation of EPO, IL-2, IL-1 1 and CD34 + cells Related.