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目的:建立榼藤子药材的质量标准,为该药材质量控制提供依据。方法:以榼藤子中主要活性成分榼藤子苷、榼藤子酰胺A-O-β-D-吡喃葡萄糖苷为指标,分别采用TLC和HPLC进行定性鉴别和含量测定研究;参照《中国药典》2010年版附录相关方法,对榼藤子药材的水分、灰分及醇溶性浸出物进行测定。结果:TLC鉴别分离良好,斑点清晰。HPLC含量测定方法学结果表明榼藤子苷在0.014~2.747 g·L-1具有良好的线性关系,r=0.999 6(n=9),平均回收率为101.06%,RSD 0.90%(n=6);榼藤子酰胺A-O-β-D-吡喃葡萄糖苷在0.002~0.452 g·L-1具有良好的线性关系,r=0.999 7(n=9),平均回收率为101.52%,RSD 1.09%(n=6);21批样品含榼藤子苷质量分数为5.12%~9.24%,榼藤子酰胺A-β-D-吡喃葡萄糖苷的质量分数为0.55%~2.17%。醇溶性浸出物为30.9%~45.2%,水分为6.6%~8.6%,总灰分为2.4%~2.9%。结论:建立的方法可用于榼藤子药材的质量控制。
OBJECTIVE: To establish the quality standard of Goto ramen and provide basis for its quality control. Methods: The main active components in the rattan rattan, AO-β-D-glucopyranoside as the indicator, respectively, by TLC and HPLC qualitative identification and determination of content; reference “Chinese Pharmacopoeia” 2010 edition Appendix Related methods, the rattan rattan herbs moisture, ash and alcohol-soluble extract was determined. Results: TLC identification of good separation, the spots clear. The method of HPLC determination showed that there was a good linear relationship between the content of ginsenoside and 0.014 ~ 2.747 g · L-1, r = 0.999 6 (n = 9), the average recovery was 101.06% and the RSD was 0.90% (R = 0.999 7, n = 9), the average recovery was 101.52% and the RSD was 1.09%. The average recovery of AO-β-D-glucopyranoside was 0.002-0.452 g · L -1 n = 6); 21 batches of samples contained 5.12% -9.24% of the content of anthocyanin, and 0.55-2.17% of the A. niger amide A-beta-D-glucopyranoside. Alcohol-soluble extract of 30.9% to 45.2%, moisture 6.6% ~ 8.6%, total ash 2.4% ~ 2.9%. Conclusion: The established method can be used for the quality control of Goto plant.