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采用两水平部分因子设计(23 Fractional Factorial),选取发酵温度、接种量、初始糖浓度为自变量考察因素,以最终发酵乙醇浓度和9 h生物量浓度为响应值,考察耐高温高浓度酵母的燃料乙醇发酵能力,并对实验数据进行二次模型变异分析(Analysis of variance,ANOVA),建立了三元二次方程数学模型.对方程求解得到,发酵温度为36.9℃,初始发酵糖浓度为280 g/L,接种量为9.4%时,最终的燃料乙醇浓度为131.0 g/L.验证实验结果显示,该酵母在37℃,初始发酵糖浓度为280 g/L,接种量为9.4%时,在48 h发酵得到129.9 g/L的乙醇,指标存在一定的先进性,证明了模型可靠有效.图2表3参13
The factors of fermentation temperature, inoculum size and initial sugar concentration were selected as independent variables by two-level fractional factorial design (23 Fractional Factorial). The final fermentation ethanol concentration and 9-h biomass concentration were used as responses to investigate the effects of high temperature and high concentration of yeast Fuel ethanol fermentation ability, and the experimental data were analyzed by Analysis of Variance (ANOVA), a mathematical model of ternary quadratic equation was established.The equation was solved, the fermentation temperature was 36.9 ℃, the initial concentration of fermented sugar was 280 g / L, the final fuel ethanol concentration was 131.0 g / L when the inoculum size was 9.4% .The validation results showed that when the initial fermentation sugar concentration was 280 g / L at 37 ℃ and the inoculum size was 9.4% After 48 h of fermentation, 129.9 g / L of ethanol was obtained, indicating that the model has certain advanced features, which proves that the model is reliable and effective.