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茶树抗云纹叶枯病鉴定的苗期接种试验,既能较客观地反映茶树田间抗病性的强弱,又能使抗病性在苗期表现出来,早期就获得鉴定结果,对抗病育种研究具实践意义,现将对30份茶资源抗云纹叶枯病的苗期鉴定试验结果作一报道。 材料与方法 (一)材料 1.供试茶苗:1年生和2年生塑料营养钵扦插苗,扦插材料来自国家茶树种质资源圃的30份资源材料。 2. 供试病原菌:从茶园中采集具有云纹叶枯病典型病斑的病叶,经室内分离纯化,用黄豆培养基培养获得病原菌分生孢子。 (二)方法 1.创伤:用不锈钢剪刀剪去芽下第2叶以下叶片
The seedling inoculation experiment of identification of resistance to curly leaf blight of tea tree can not only objectively reflect the strength of disease resistance in the field of tea tree but also show the disease resistance in the seedling stage and obtain the identification result early, Breeding research with practical significance, now 30 tea germplasm resources of resistance to the germination of the test results made a report. Materials and methods (a) material 1 for test tea seedlings: 1-year-old and 2-year-old plastic nutrition Bowl cutting seedlings, cuttings from the National Tea Germplasm Resources 30 resource materials. 2. for the test pathogens: collected from the tea plant disease with the typical lesion leaf spot blight, isolated and purified by the indoor culture with soybean medium obtained pathogen conidia. (B) Method 1. Trauma: Use stainless steel scissors to cut off the leaves under the second leaf following