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[目的]观察黄芪多糖对血管性痴呆大鼠学习记忆能力的影响,并探讨其机制.[方法]用两侧颈总动脉永久性结扎法制作血管性痴呆大鼠模型,并随机分成对照组和黄芪多糖组.两组分别灌胃给予生理盐水和黄芪多糖水溶液30d后,利用脑部微量透析法和高效液相色谱法测定大鼠海马区细胞外液中的天冬氨酸、谷氨酸、谷氨酰胺、甘氨酸及牛磺酸水平,并进行Morris水迷宫实验.[结果]与对照组相比较,黄芪多糖组的逃避潜伏期明显缩短(P<0.05),穿越原站台区次数明显增加,而且海马区谷氨酸、谷氨酰胺、牛磺酸及甘氨酸水平均显著降低(P<0.05).[结论]黄芪多糖增强血管性痴呆模型大鼠空间学习记忆能力,其作用机制可能与降低海马区几种氨基酸物质水平有关.
[Objective] To observe the effect of astragalus polysaccharides on the learning and memory abilities of vascular dementia rats and to explore its mechanism. [Methods] Rat model of vascular dementia was made by permanent ligation of both common carotid arteries and randomly divided into control group and control group Astragalus polysaccharide group.The two groups were given intragastric administration of saline and astragalus polysaccharide solution 30d, using brain microdialysis and high performance liquid chromatography in rat hippocampus extracellular fluid aspartate, glutamic acid, Glutamine, glycine and taurine levels, and Morris water maze test. [Results] Compared with the control group, the escape latency of Astragalus polysaccharide group was significantly shortened (P <0.05), the number of crossing the original platform area increased significantly, and The levels of glutamate, glutamine, taurine and glycine in hippocampus of hippocampus were significantly decreased (P <0.05). [Conclusion] Astragalus polysaccharide can enhance spatial learning and memory of vascular dementia model rats, which may be related to the decrease of hippocampus Several amino acid levels related.