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目的:对黔产不同产区苦参根、茎、叶与经纬度、海拔、平均湿度、平均温度进行相关性分析,了解不同生态环境下苦参不同部位生物碱含量累积。方法:采用紫外分光光度法测定苦参的根、茎、叶生物总碱的含量,利用相关性分析(CA)法研究其生物碱累积差异与生态因子的相关性。结果:苦参不同部位生物碱含量大小依次为根>叶>茎,其中最高的是毕节市大方县的苦参根、茎、叶生物碱质量分数之和为20.45μg·g~(-1);苦参不同部位受到生态因子的不同程度影响。结论:苦参根、茎、叶生物碱含量在不同的产区有显著差异,生态因子中相对湿度和年均温对叶生物碱含量影响最大,该结果可为中药苦参的规范化、标准化栽培管理和目标化种植提供理论依据,寻找苦参优质产区及为非药用部位综合利用提供理论依据。
OBJECTIVE: To analyze the correlation between the root, stem, leaf and the latitude, longitude, latitude, altitude, average humidity and average temperature of Sophora flavescens in different producing areas in Guizhou Province, and to understand the accumulation of alkaloids in different parts of Sophora flavescens in different ecological environments. Methods: The contents of total alkaloids in roots, stems and leaves of Sophora flavescens were determined by ultraviolet spectrophotometry. Correlation analysis (CA) was used to study the correlation between the accumulation of alkaloids and the ecological factors. Results: The content of alkaloids in different parts of Sophora flavescens was root> leaf> stem, and the highest content of alkaloids in root, stem and leaf of Sophora flavescens in Dafang county was 20.45μg · g ~ (-1) The different parts of Sophora flavescens are affected by different degrees of ecological factors. Conclusion: The content of alkaloids in roots, stems and leaves of Sophora flavescens is significantly different in different producing areas. The relative humidity and annual average temperature of ecological factors have the greatest effect on the content of alkaloids in the leaves. The results can be standardized and standardized cultivation of Sophora flavescens Management and the goal of planting provide the theoretical basis for finding the high quality production areas of Sophora flavescens and provide a theoretical basis for the comprehensive utilization of non-medicinal parts.