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为了解福建省甲型H1N1流感病毒基因变异特征和规律,对2009~2012年福建省分离的14株甲型H1N1流感病毒进行了全基因序列分析。结果发现,所有的毒株均是典型的低致病性流感病毒,对金刚烷胺类药物耐药,对神经氨酸酶抑制剂敏感。所有毒株与A/California/07/2009(H1N1)疫苗株保持高度同源,8个节段基因同源性均在98.2%以上。相比之下,福建省2012年的流感毒株抗原变异程度较大,其中A/Fujiangulou/SWL1155/2012毒株HA基因发生11个氨基酸位点改变,其中H138R、L161I、S185T和S203T位点的变异分别涉及Ca、Sa和Sb抗原决定簇。监测显示,疫苗对福建省人群保护效果较好,但福建省2012年毒株相对疫苗株已经出现抗原漂移,应进一步密切关注其变异情况。
In order to understand the characteristics and rules of the genetic variation of the H1N1 influenza virus in Fujian Province, the complete genome sequence analysis of 14 strains of H1N1 influenza viruses isolated in Fujian Province from 2009 to 2012 was conducted. The results showed that all the strains are typical low pathogenic influenza virus, amantadine resistance, sensitive to neuraminidase inhibitors. All strains were highly homologous to the vaccine strain A / California / 07/2009 (H1N1), and the homology of the eight segments was over 98.2%. In contrast, there was a large variation in the antigenic variation of influenza strains in 2012 in Fujian Province. The HA gene of A / Fujiangulou / SWL1155 / 2012 changed 11 amino acid residues, of which, H138R, L161I, S185T and S203T Variants involved Ca, Sa and Sb epitopes respectively. Surveillance shows that the vaccines have a good protective effect on the population in Fujian Province. However, the antigenic drift of the vaccine strains has already appeared in Fujian Province in 2012, and its variation should be closely monitored.