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目的:观察扶正除疫颗粒对A型H1N1流感病毒感染小鼠T细胞亚群的影响,探讨其抗A型H1N1流感病毒感染的作用特点和机制。方法:40只BALB/c小鼠随机分为正常对照组、病毒模型组、达菲对照组、扶正除疫组。正常对照组和病毒模型组以0.9%氯化钠溶液灌胃,另两组以达菲、扶正除疫颗粒水溶液灌胃,连续7d。除正常对照组外,其余各组于给药第3天,以FM1-6-E2滴鼻造模。每组于造模后3、6、9d分别取脾脏制备细胞悬液,采用流式细胞仪测定T细胞亚群的变化。结果:各组与同期病毒模型组相比,除第3天的CD3+T、第6天的CD4+T及第9天CD3+T和扶正除疫组CD4+T和百分率数值没有显著差异外,其余各组各时段的CD3+T、CD4+T、CD8+T及CD4+T/CD8+T百分率数值均有显著差异(P<0.01,P<0.05)。结论:扶正除疫颗粒对A型H1N1流感病毒感染引起的细胞免疫功能失常有一定的调节作用。提示该药物抗流感病毒的作用机制,可能是通过调整T细胞亚群的百分率及其CD4+T/CD8+T比值得以实现。
Objective: To observe the effect of Fuzheng Zhiji granule on T lymphocyte subsets in mice infected with influenza A (H1N1) virus and to explore its mechanism of action and anti-A H1N1 influenza virus infection. Methods: Forty BALB / c mice were randomly divided into normal control group, virus model group, Tamiflu control group and Fuzheng et al. The normal control group and the model group were orally gavaged with 0.9% sodium chloride solution. The other two groups were administered with Tamiflu and Fuzheng Zhuyi granule aqueous solution for 7 days. Except the normal control group, the other groups were given intranasal administration of FM1-6-E2 on the third day after administration. Spleens were prepared at 3, 6, and 9 days after model establishment, and the changes of T cell subsets were determined by flow cytometry. Results: Compared with the same virus model group, except the CD3 + T on the third day, the CD4 + T on the sixth day, the CD3 + T on the ninth day and the CD4 + T and the percentages in the Fuzheng Zhuji group (P <0.01, P <0.05). The percentages of CD3 + T, CD4 + T, CD8 + T and CD4 + T / CD8 + T in the other groups were significantly different. Conclusion: Fuzheng Chuji Granule has a certain regulatory effect on cellular immune dysfunction caused by influenza A (H1N1) virus infection. Suggesting that the drug anti-influenza virus mechanism of action may be by adjusting the percentage of T-cell subsets and its CD4 + T / CD8 + T ratio can be achieved.