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An efficient and safe gene delivery system remains a challenge in the development of gene therapy.Polycation-based gene nanoparticles are a typical non-viral gene delivery system,which are able to transfect cells in vitro and in vivo.This paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly,in which the polycation-based gene nanoparticles were loaded.Through this surface-mediated delivery system,adherent cells on the multilayer could be transfected in situ.Gene nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the DNA.DNA nanoparticles/PGA multilayers constructed on the scaffold surface could also realize in situ transfection on the adherent cells.The well-structured,easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles,which may have potential applications for gene therapy in tissue engineering and medical implants.
An efficient and safe gene delivery system remains a challenge in the development of gene therapy. Polycation-based gene nanoparticles are a typical non-viral gene delivery system, which are able to transfect cells in vitro and in vivo. This paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly, in which the polycation-based gene nanoparticles were loaded. Through this surface-mediated delivery system, adherent cells on the multilayer could be transfected in situ. Gene nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the DNA. DNA nanoparticles / PGA multilayers constructed on the scaffold surface also also in situ transfection on the adherent cells.The well-structured, easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles, which may have potential applications for gene therapy in tissu e engineering and medical implants.