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目的探讨苯并[a]芘(BaP)对大鼠学习记忆及海马神经元的影响。方法将50只SD大鼠随机分为空白对照组、溶剂对照组、BaP低(2.5 mg/kg)、中(5 mg/kg)、高(10 mg/kg)剂量组,腹腔注射染毒,每天1次,持续30d;用Morris水迷宫测试学习记忆,电镜观察海马神经元超微结构。结果空白对照组、溶剂对照组和BaP低、中、高剂量组穿越平台次数分别为(12.03±2.43)、(11.35±2.68)、(9.75±1.78)、(7.63±1.67)、(4.38±1.46)次,在平台象限停留时间分别为(46.34±11.56)、(48.12±13.22)、(32.35±7.68)、(26.13±5.34)、(16.14±3.15)s,差异均有统计学意义(P<0.05);随染毒剂量的增加,BaP低、中、高剂量组大鼠的平均逃避潜伏期和第1次寻找平台的潜伏期均延长,平均有效策略百分比、穿越平台的次数均减少,在平台象限停留时间均缩短,差异均有统计学意义(P<0.05);电镜观察结果显示,BaP低剂量组海马神经元胞体浓缩变性,核膜皱缩变形;中剂量组出现线粒体肿胀;高剂量组出现高尔基扩张。结论 BaP亚慢性染毒对大鼠具有一定的神经行为毒性。
Objective To investigate the effects of benzo [a] pyrene (BaP) on learning and memory and hippocampal neurons in rats. Methods Fifty Sprague Dawley rats were randomly divided into blank control group, solvent control group, BaP low (2.5 mg / kg), medium (5 mg / kg) and high (10 mg / Once a day for 30 days. Morris water maze test learning and memory, electron microscopy ultrastructure of hippocampal neurons. Results The number of crossing platform of the control group, the solvent control group and the BaP low, medium and high dose groups were (12.03 ± 2.43), (11.35 ± 2.68), (9.75 ± 1.78), (7.63 ± 1.67), (4.38 ± 1.46 ), And the stay time in the platform quadrant was (46.34 ± 11.56), (48.12 ± 13.22), (32.35 ± 7.68), (26.13 ± 5.34) and (16.14 ± 3.15) s respectively, with significant differences between the two groups (P < 0.05). With the increase of the dose, the average escape latency of BaP low, medium and high dose groups and the latency of finding the first platform were prolonged. The average effective strategy percentage and the number of crossing the platform all decreased. (P <0.05). Electron microscopy results showed that the BaP low concentration group had degeneration and degeneration of nuclear bodies in the hippocampal neurons, mitochondria swelling in the middle dose group, and high dose group Gorky expansion. Conclusion Subchronic exposure to BaP has neurobehavioral toxicity in rats.