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山葡萄（Vitis amurensis Rupr.）一般为雌雄异株,我国是其主要原产地。山葡萄在其漫长的进化过程中,不仅有花型上的差别,而且在植株形态、果实经济性状、抗病力等方面均有差异。在种质资源评价过程中,主要依据上述指标进行,但这些指标易受环境条件的影响,很难对种质进行准确评价。 RAPD技术已成功地应用于葡萄种、品种的鉴别,但在山葡萄上的应用则未见报道。本研究的目的在于以RAPD技术阐明山葡萄的种内变异。1 材料与方法1.1 材料 取山葡萄73064（（?））左山二（（?））、75023（（?））、双丰（（?））、左山一（（?））、73093（（?））、73066（（?））的休眠枝条,解除休眠后水插,取幼叶提取基因组DNA。试验于1997～1998年在北京市农林科学院林果所进行。
Vitis amurensis Rupr. Is generally dioecious and my country is its main origin. In its long evolution process, grapevine not only has the difference of flower pattern, but also has the difference in plant morphology, fruit economic traits, disease resistance and so on. Germplasm resources evaluation process, mainly based on the above indicators, but these indicators are susceptible to environmental conditions, it is difficult to accurately evaluate the germplasm. RAPD technology has been successfully applied to the identification of grape species and varieties, but the application on the grape has not been reported. The purpose of this study is to elucidate the intraspecies variation of grapevine by RAPD technique. 1 Materials and methods 1.1 Materials Take grapevine 73064 ((?)) Zuoshan II ((?)), 75023 (?), Shuangfeng (?), Zuoshan one (?), 73093 ( ?)), 73066 ((?)) Of the dormant branches, remove the dormant water plug, take the leaves to extract genomic DNA. The experiment was carried out in Beijing Academy of Agriculture and Forestry Sciences from 1997 to 1998.