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目的对Buerger’s病患者血管中的髓样分化因子(myeloid differentiation factor88,My D88)进行检测,观察My D88在Buerger’s病患者血管中的表达及分布。方法收集2013年7月至2015年7月我院Buerger’s病患者血管标本9例,同期非Buerger’s病(肿瘤、外伤)患者血管标本12例为对照组。运用RT-PCR、Western blot技术检测2组血管中My D88的表达水平。同时运用免疫组化、免疫荧光技术检测Buerger’s病血管中My D88的表达及分布规律。结果 RT-PCR、Western blot结果显示,与对照组相比,Buerger’s病患者血管组织中My D88的m RNA和蛋白表达水平明显升高,具有显著统计学意义(P<0.01)。免疫组化、免疫荧光结果显示,在Buerger’s病患者血管组织中,My D88主要表达于内皮细胞和平滑肌细胞。结论 Buerger’s病患者血管组织中My D88基因和蛋白表达水平明显升高,且主要表达于血管平滑肌细胞和内皮细胞。这些结果表明My D88可能与Buerger’s病的发病密切相关,TLRs-My D88信号通路在Buerger’s病的发病机制中可能发挥着重要作用。
Objective To detect Myeloid differentiation factor 88 (My D88) in blood vessels of Buerger’s disease patients and to observe the expression and distribution of My D88 in blood vessels of Buerger’s disease patients. Methods Nine patients with Buerger’s disease in our hospital from July 2013 to July 2015 were collected, and 12 patients with non-Buerger’s disease (tumor and trauma) were selected as the control group. The expression of My D88 in two groups of blood vessels was detected by RT-PCR and Western blot. Meanwhile, immunohistochemistry and immunofluorescence were used to detect the expression and distribution of My D88 in Buerger’s disease blood vessels. Results The results of RT-PCR and Western blot showed that the mRNA and protein levels of My D88 in Buerger’s disease patients were significantly increased compared with the control group (P <0.01). Immunohistochemistry and immunofluorescence showed that My D88 was mainly expressed in endothelial cells and smooth muscle cells in Buerger’s disease patients. Conclusions The expression of My D88 gene and protein in Buerger’s disease patients’ vascular tissues was significantly increased, and mainly expressed in vascular smooth muscle cells and endothelial cells. These results indicate that My D88 may be closely related to the pathogenesis of Buerger’s disease, and TLRs-My D88 signaling pathway may play an important role in the pathogenesis of Buerger’s disease.